Human DA(Dopamine) ELISA Kit

Human DA(Dopamine) ELISA Kit

AO-06-ELK9845-96T

Human DA(Dopamine) ELISA Kit 96T

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Product Overview

Cat.No ELK9845
Product Name Human DA(Dopamine) ELISA Kit
Reactivity Human
Alternative Names 2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
Assay Type Competitive Inhibition
Sensitivity 4.53 pg/mL
Standard 1000 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Type serum, plasma and other biological fluids
Assay Length 2h
Research Area Signal transduction;Cardiovascular biology;Reproductive science;Neuro science;
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK9845-b-1.jpg
: AO-06-ELK9845-96T
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