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Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit
AO-06-ELK0755-48T
Product Overview
| Cat.No | ELK0755 |
| Product Name | Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | 15-Deoxy-delta 12,14-prostaglandin J2 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.23 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Endocrinology;Pulmonology;Hepatology;Hormone metabolism; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 15d-PGJ2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 15d-PGJ2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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: AO-06-ELK0755-48T
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