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Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit
AO-06-ELK0650-48T
Product Overview
| Cat.No | ELK0650 |
| Product Name | Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | Anti-Insulin; Antiinsulin; Insulin Antibodies |
| Assay Type | Sandwich |
| Sensitivity | 0.32 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | Serum, plasma and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Signal transduction;Endocrinology;Autoimmunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0650-48T
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