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Mouse BAFF/CD257(B-Cell Activating Factor) ELISA Kit
AO-06-ELK10525-48T
Product Overview
| Cat.No | ELK10525 |
| Product Name | Mouse BAFF/CD257(B-Cell Activating Factor) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF |
| Assay Type | Sandwich |
| Sensitivity | 0.067 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK10525-48T
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