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Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit
AO-06-ELK0019-96T
Product Overview
| Cat.No | ELK0019 |
| Product Name | Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | ERK; ERK-2; ERK2; ERT1; MAPK2; NS13; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK |
| Assay Type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0019-96T
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