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Mouse HMGB-1(High Mobility Group Protein B1) Microsample ELISA Kit
AO-06-ELK10273MS-48T
Product Overview
| Cat.No | ELK10273MS |
| Product Name | Mouse HMGB-1(High Mobility Group Protein B1) Microsample ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1 |
| Assay Type | Sandwich |
| Sensitivity | 0.063 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Infection immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK10273MS-48T
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