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Mouse IL-27(Interleukin 27) Microsample ELISA Kit
AO-06-ELK9612MS-48T
Product Overview
| Cat.No | ELK9612MS |
| Product Name | Mouse IL-27(Interleukin 27) Microsample ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | IL-27; IL-27A; IL27p28; IL30; MGC71873; p28; |
| Assay Type | Sandwich |
| Sensitivity | 19.12 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Immunology;Cancer |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9612MS-48T
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