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Mouse KNG1(Kininogen 1) ELISA Kit
AO-06-ELK10547-48T
Product Overview
| Cat.No | ELK10547 |
| Product Name | Mouse KNG1(Kininogen 1) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | BDK; KNG; HMWK; Alpha-2-Thiol Proteinase Inhibitor; Bradykinin; Kallidin; Fitzgerald factor; High molecular weight kininogen; Williams-Fitzgerald-Flaujeac factor; Ile-Ser-Bradykinin |
| Assay Type | Sandwich |
| Sensitivity | 0.67 µg/mL |
| Standard | 100 µg/mL |
| Detection Range | 1.57-100 µg/mL |
| Sample Type | plasma |
| Assay Length | 3.5h |
| Research Area | Hematology; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KNG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KNG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KNG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KNG1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK10547-48T
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