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Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit
AO-06-ELK9883-96T
Product Overview
| Cat.No | ELK9883 |
| Product Name | Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | Smad 2/3 |
| Assay Type | Sandwich |
| Sensitivity | 0.059 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates |
| Assay Length | 3.5h |
| Research Area | Signal transduction;Apoptosis;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Smad2/3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9883-96T
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