Mouse THP(Tamm–Horsfall Glycoprotein) ELISA Kit

Mouse THP(Tamm–Horsfall Glycoprotein) ELISA Kit

ELK Biotechnology
AO-06-ELK6195-96T

Mouse THP(Tamm–Horsfall Glycoprotein) ELISA Kit 96T

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Product Overview

Cat.No ELK6195
Product Name Mouse THP(Tamm–Horsfall Glycoprotein) ELISA Kit
Reactivity Mouse
Alternative Names THP; Uromucoid,Tamm-Horsfall Glycoprotein; Tamm-Horsfall urinary glycoprotein; Uromodulin; UMOD
Assay Type Sandwich
Sensitivity 0.056 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type serum, plasma, urine and other biological fluids
Assay Length 3.5h
Research Area Kidney biomarker;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse THP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse THP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse THP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse THP in the samples is then determined by comparing the OD of the samples to the standard curve.

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: AO-06-ELK6195-96T
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Hurry! only 10 items left in stock.

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