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Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
AO-06-ELK9710-48T
Product Overview
| Cat.No | ELK9710 |
| Product Name | Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | TPO Ab; TPO-Ab |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.25 IU/mL |
| Standard | 80 IU/mL |
| Detection Range | 1.25-80 IU/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
| Research Area | Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
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