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Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit
AO-06-ELK9616-96T
Product Overview
| Cat.No | ELK9616 |
| Product Name | Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | ACP5;TRAP5b;SPENCDI |
| Assay Type | Sandwich |
| Sensitivity | 0.46 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Immunology;Cardiovascular |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9616-96T
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