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Antibody Product Overview
| ELK.No | EM1052 |
| Product name | p53 Mouse mAb |
| Reactivity | Human |
| Applications | WB, IHC, IF |
| Other name | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 7157 |
| Human Swiss-Prot | P04637 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic Peptide |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:2,000 IHC 1:200 IF 1:100-200 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 53kDa |
| Background | p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. |
| Function | |
| Subcellular location | Cytoplasm; Cytoplasm. Nucleus |
| Expression |
Additional Images
Western blot analysis of 293T, with p53 mAb diluted at 1) 1:1,000 2) 1:2,000
IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb（6C4）diluted at 1:200.
IF analysis of Hela with EM1052(Left) and DAPI (Right) diluted at 1:100.
: EM1052-100
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