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Antibody Product Overview
| ELK.No | EM1136 |
| Product name | eIF4A1 Mouse mAb |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Other name | DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1973 |
| Human Swiss-Prot | P60842 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic Peptide |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 48kDa |
| Background | The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression. |
| Function | |
| Subcellular location | Cytosol,Extracellular region or secreted |
| Expression |
Additional Images
Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000.
IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（EM1136）Mouse mAb diluted at 1:200.
: EM1136-100
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