Anti-Ki67 Rabbit pAb, Primary Antibody

Anti-Ki67 Rabbit pAb, Primary Antibody

AO-03-GB121142-10

Anti-Ki67 Rabbit pAb, Primary Antibody

Volume : 10 µL
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Anti-Ki67 Rabbit pAb

Description

Ki67 rabbit polyclonal antibody

Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Ki67

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Uniprot ID

E9PVX6


Applications

 

IHC/IF

Mouse, Rat

1: 500-1: 1200

colon, placenta, testis


Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .


Images

01 GB111141_ki67_459219073102 M placenta d1000_31.1x_IHC.jpg

IHC analysis of ki67 (GB111141).

Sample: Mouse placenta (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

02 GB111141_Ki67_5405_M subcutaneous transplant tumor(H22) d500_40.0x_IHC.jpg

IHC analysis of Ki67 (GB111141).

Sample: Mouse subcutaneous transplant tumor(H22) (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

03 GB111141_ki67_459219073102 R colon d750_40.0x_IHC.jpg

IHC analysis of ki67 (GB111141).

Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 750, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

04 GB111141_ki67_4592_M testis d600_20.0x_IF.jpg

IF analysis of ki67 (GB111141).

Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 600, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

05 GB111141_ki67_459219073102 R colon d600_20.0x_IF.jpg

IF analysis of ki67 (GB111141).

Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 600, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.


Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

: AO-03-GB121142-10
: 10 Items
Hurry! only 10 items left in stock.

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