Anti-PCNA Rabbit pAb, Primary Antibody
Anti-PCNA Rabbit pAb
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Protein full name |
Proliferating cell nuclear antigen |
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Synonyms |
PCNA, ATLD2, proliferating cell nuclear antigen |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
|
Uniprot ID |
P17918 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus |
|
Predicted MW. / Observed MW. |
29 kDa / 36 kDa |
Applications
|
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
HeLa, SW480, B16 |
|
IHC/IF |
Human, Mouse, Rat |
1: 200-1: 1000 |
intestinal cancer, liver cancer, lung cancer |
|
ICC/IF |
Human, Mouse, Rat |
1:200-1: 400 |
HeLa |
|
IP |
Human, Mouse, Rat |
1: 50-1: 200 |
HeLa cells |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
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Western blot analysis of PCNA (GB11010) at dilution of 1: 1000 |
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Western blot analysis of PCNA (GB11010) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded human liver cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunofluorescence of paraffin embedded rat testis using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunoprecipitation of HeLa cells lysate using PCNA（IP: GB11010, 1: 100; Detection: GB12010, 1: 2000） |
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ICC analysis of PCNA(GB11010)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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