Anti-Vimentin Rabbit pAb, Primary Antibody

Anti-Vimentin Rabbit pAb, Primary Antibody

AO-03-GB121142-10

Anti-Vimentin Rabbit pAb, Primary Antibody

Volume : 10 µL
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Anti-Vimentin Rabbit pAb

Description

Vimentin rabbit polyclonal antibody

Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse Vimentin

Isotype

IgG

Purity

Affinity purification

Predicted MW.

54 kDa

Observed MW.

54 kDa

Subcellular location

Cytoplasm, Nucleus

Uniprot ID

P08670, P20152, P31000

Applications

Applications

Species

Dilution

Positive Sample

WB

Human,Mouse,Rat

1: 500-1: 1000

lung

IHC/IF

Human,Mouse,Rat

1: 1500-1: 3000

breast cancer, thymus, lymph, colon, lung, kidney, ovary, spleen

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Images

1643163908229013829.png

WB analysis of Vimentin (GB111308).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

1643163907171122068.png

IHC analysis of Vimentin (GB111308).

Sample: Human breast cancer (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1643163907179420679.png

IHC analysis of Vimentin (GB111308).

Sample: Human breast cancer (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1600, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1643163907200301623.png

IHC analysis of Vimentin (GB111308).

Sample: Mouse immune spleen (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1643163907773782075.png

IHC analysis of Vimentin (GB111308).

Sample: Mouse placenta (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1643163907982122038.png

IHC analysis of Vimentin (GB111308).

Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1643163908454314488.png

IHC analysis of Vimentin (GB111308).

Sample: Rat lymph (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1643163908662365191.png

IF analysis of Vimentin (GB111308).

Sample: Human ovary cancer (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1600, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

1643163908907427347.png

IF analysis of Vimentin (GB111308).

Sample: Human colon (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

1643163909095309124.png

IF analysis of Vimentin (GB111308).

Sample: Mouse kindey (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

1643163909297266883.png

IF analysis of Vimentin (GB111308).

Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

1643163909518864368.png

IF analysis of Vimentin (GB111308).

Sample: Rat ovary (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

1643163909807562842.png

IF analysis of Vimentin (GB111308).

Sample: Rat spleen (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB121142-10
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Hurry! only 10 items left in stock.

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