Anti-Vimentin Rabbit pAb, Primary Antibody

Anti-Vimentin Rabbit pAb, Primary Antibody

AO-03-GB11192-10

Anti-Vimentin Rabbit pAb, Primary Antibody

Volume : 10 µL
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Anti-Vimentin Rabbit pAb

Description

Vimentin rabbit polyclonal antibody

Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse vimentin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

57 kDa

Observed MW.

57 kDa

Uniprot ID

P08670,P20152,P31000

reactivity (External validation)

Pelodiscus sinensis


Applications

Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 500-1: 1000

lung

IHC/IF

Human, Mouse, Rat

1: 200-1: 1000

colon, lung cancer, ovarian cancer

ICC/IF

Human, Mouse

1: 200-1: 1000

HeLa cell, NIH/3T3 cell


Background

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.


Images

1627458637601892307.png

Western blot analysis of Vimentin (GB11192) at dilution of 1: 500

1627458638013541946.png

Immunohistochemistry of paraffin embedded human colon using vimentin (GB11192) at dilution of 1: 500 (400x lens)

1627458637677145285.png

Immunohistochemistry of paraffin embedded human breast cancer using vimentin (GB11192) at dilution of 1: 500 (200x lens)

1627458637666029716.png

Immunohistochemistry of paraffin embedded human breast cancer using vimentin (GB11192) at dilution of 1: 400 (400x lens)

1627458638242705475.png

Immunofluorescence of paraffin embedded human colon using vimentin (GB11192) at dilution of 1: 500 (200x lens)

1627458638492863313.png

Immunofluorescence of paraffin embedded human ovarian cancer using vimentin (GB11192) at dilution of 1: 400 (200x lens)

1627458638738829767.png

Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cell using Vimentin (GB11192) at dilution of 1: 800

1627458639026704181.png

Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell using Vimentin (GB11192) at dilution of 1: 800


Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

: AO-03-GB11192-10
: 10 Items
Hurry! only 10 items left in stock.

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