Recombinant Anti-P53 Rabbit MAb, Primary Antibody

Recombinant Anti-P53 Rabbit MAb, Primary Antibody

AO-03-GB15627-50

Recombinant Anti-P53 Rabbit MAb, Primary Antibody

Volume : 50 µL
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Recombinant Anti-P53 Rabbit MAb

Protein full name

Cellular tumor antigen p53

Synonyms

TP53, P53

Immunogen

Recombinant protein corresponding to Mouse p53

Uniprot ID

P04637,P02340,P10361

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Endoplasmic reticulum, Mitochondrion

Predicted MW. / Observed MW.

44 kDa / 53 kDa

Applications

WB

Human

1: 1000-1: 3000

293T,U-251 MG,T-47D

IHC/IF

Human

1: 500-1: 1000

ovarian cancer

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases.

Images

01 GB15627_P53_重组H190_d1000_WB.jpg

WB analysis of P53 (GB15627).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

02 GB15627_P53_ZCR15626_d1000_H carcinoma esophageal_40.0x_IHC.jpg

IHC analysis of P53(GB15627).

Sample: Human esophageal carcinoma,4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

03 GB15627_P53_ZCR15626_d1000_H cancer ovarian_40.0x_IHC.jpg

IHC analysis of P53(GB15627).

Sample: Human ovarian cancer,4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

Storage

Storage             Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB15627-50
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Hurry! only 10 items left in stock.

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