Anti-BrdU Mouse mAb, Primary Antibody

Anti-BrdU Mouse mAb, Primary Antibody

AO-03-GB12051-50

Anti-BrdU Mouse mAb, Primary Antibody

Volume : 50 µL
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Anti-BrdU Mouse mAb

Protein full name

Bromodeoxyuridine

Synonyms

Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse BrdU

Isotype

IgG1,κ

Purity

Affinity purification

Subcellular location

Nucleus

Applications

 

IHC/IF

All species

1: 100-1: 500

jejunum, neonatal mouse's brain

Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.

Images

Picture 1.png

IHC analysis of Brdu (GB12051).
Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: 2M HCL in pure water，37℃, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
Picture 2.png

IHC analysis of Brdu (GB12051).
Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: 2M HCL in pure water，37℃, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
Picture 3.png

IF analysis of Brdu (GB12051).
Sample: Mouse neonatal mouse's brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB12051-50
: 10 Items
Hurry! only 10 items left in stock.

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