Recombinant Anti-p38 Rabbit mAb, Primary Antibody

Recombinant Anti-p38 Rabbit mAb, Primary Antibody

AO-03-GB154685-50

Recombinant Anti-p38 Rabbit mAb, Primary Antibody

Volume : 50 µL
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Recombinant Anti-p38 Rabbit mAb

Protein full name

Mitogen-activated protein kinase 14

Synonyms

Mitogen-activated protein kinase p38 alpha, MAP kinase 14, CRK1, Mapk14, MAP kinase p38 alpha, Crk1, Csbp1, Csbp2, P38

Immunogen

KLH conjugated Synthetic peptide corresponding to Human p38

Uniprot ID

Q16539,O15264,P53778,Q15759

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW. / Observed MW.

38 kDa / 40 kDa

Applications

WB

Human, Mouse, Rat

1: 1000-1: 2000

C6, HT-29, C6+UV, HT-29+UV

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

testis, subcutaneous nude mouse model of 4T1 cel

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.

Images

01 GB154685_p38_重组H218 M007_d1000_WB.png

WB analysis of p38 (GB154685).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02 GB154685_P38_H297_M007230901_d500_H gastric carcinoma_25.3x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03 GB154685_P38_H297_M007230901_d500_M subcutaneous nude mouse model of 4T1 cell_40.0x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Mouse subcutaneous nude, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04 GB154685_P38_H297_M007230901_d500_M testis_31.3x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Mouse testis, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05 GB154685_P38_H297_M007230901_d500_R testis_20.0x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Rat testis, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06 GB154685_P38_H297_M007230901_d1000_H cervical carcinoma_25.5x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07 GB154685_P38_H298_M007230902_d500_H gastric carcinoma_20.0x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

08 GB154685_P38_H298_M007230902_d500_M subcutaneous nude mouse model of 4T1 cell_28.3x_IHC.-WM.png

IHC analysis of P38(GB154685).

Sample: Mouse subcutaneous nude mouse model of 4T1 cell, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

09 GB154685_P38_H298_M007230902_d500_M testis_24.6x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Mouse testis, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

10 GB154685_P38_H298_M007230902_d500_R testis_25.3x_IHC-WM.png

IHC analysis of P38(GB154685).

Sample: Rat testis, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0)(G1201), pressure cooker 2min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage             Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
: AO-03-GB154685-50
: 10 Items
Hurry! only 10 items left in stock.

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