Recombinant Anti-Bcl-2 Rabbit mAb, Primary Antibody

Recombinant Anti-Bcl-2 Rabbit mAb, Primary Antibody

AO-03-GB154380-50

Recombinant Anti-Bcl-2 Rabbit mAb, Primary Antibody

Volume : 50 µL
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Recombinant Anti-Bcl-2 Rabbit mAb

Protein full name

Apoptosis regulator Bcl-2

Synonyms

BCL2, Bcl-2, PPP1R50, B-cell CLL/lymphoma 2, apoptosis regulator

Immunogen

Recombinant protein corresponding to Human Bcl-2

Uniprot ID

P10415

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW. / Observed MW.

26 kDa / 26 kDa


Applications

WB

Human, Mouse

1: 500-1: 1000

NIH/3T3, spleen, Jurkat

IHC/IF

Human, Mouse

1: 500-1: 1000

tonsil,lymph node


Background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells . Regulates cell death by controlling the mitochondrial membrane permeability . Appears to function in a feedback loop system with caspases . Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.


Images

01 GB154380_Bcl-2_M017223121403_d200_WB_WM.png

WB analysis of Bcl-2(GB154380).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02 GB154380_Bcl-2_M017_d100_H tonsil_40.0x_IHC_WM.png

IHC analysis of Bcl-2(GB154380).

Sample: Human tonsil, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03 GB154380_Bcl-2_M017_d100_M lymph node_40.0x_IHC_WM.png

IHC analysis of Bcl-2(GB154380).

Sample: Mouse lymph node, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04 GB154380_Bcl-2_M017_d1000_M spleen_40.0x_IHC_WM.png

IHC analysis of Bcl-2(GB154380).

Sample: Mouse spleen, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.


Storage

Storage             Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

: AO-03-GB154380-50
: 10 Items
Hurry! only 10 items left in stock.

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