Anti-mTOR Rabbit pAb, Primary Antibody

Anti-mTOR Rabbit pAb, Primary Antibody

AO-03-GB111839-50

Anti-mTOR Rabbit pAb, Primary Antibody

Volume : 50 µL
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Anti-mTOR Rabbit pAb

Protein full name

Serine/threonine-protein kinase mTOR

Synonyms

FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, mTOR

Immunogen

KLH conjugated Synthetic peptide corresponding to Human mTOR

Uniprot ID

P42345

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Predicted MW. / Observed MW.

289 kDa / 289 kDa

 

Applications

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

heart, liver, brain

IHC/IF

Mouse, Rat

1: 200-1: 600

brain

 

Background

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications.

Images

01 GB111839_6593 mTOR_WB.jpg

WB analysis of mTOR (GB111839)
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02 GB111839_20200601_mTOR_6593_659320041902 M brain d300_20.0x_IHC.jpg

IHC analysis of mTOR (GB111839)

Sample: Mouse brain 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.

Primary antibody: 1: 300, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03 GB111839_20200601_mTOR_6593_659320041902 R brain d300_20.0x_IHC.jpg

IHC analysis of mTOR (GB111839)

Sample: Rat brain 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.

Primary antibody: 1: 300, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB111839-50
: 10 Items
Hurry! only 10 items left in stock.

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