Recombinant Anti-beta Catenin antibody (Rabbit mAb), Primary Antibody

Recombinant Anti-beta Catenin antibody (Rabbit mAb), Primary Antibody

AO-03-GB15015-50

Recombinant Anti-beta Catenin antibody (Rabbit mAb)

Volume : 50 µL
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Recombinant Anti-beta Catenin antibody

Protein full name

Catenin beta-1

Synonyms

Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin, Cadherin associated protein, armadillo, catenin beta 1, CATNB

Immunogen

Recombinant protein corresponding to Mouse beta Catenin

Uniprot ID

P35222

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton, Nucleus, Synapse, Cell projection, Cell membrane

Predicted MW. / Observed MW.

85 kDa / 92 kDa

Applications

WB

Human, Mouse, Rat

1: 500-1: 1000

A549,A-431,lung,brain

IHC/IF

Human, Mouse, Rat

1: 1000-1: 2000

esophageal carcinoma,stomach

Background

β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to γ-catenin, also known as plakoglobin.

Images

01 GB15015_beta Catenin_重组H188_d2000_WB.png

WB analysis of beta Catenin (GB15015).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02 GB15015_-catenin_ZCR17021_d1000_H esophageal carcinoma_40.0x_IHC.png

IHC analysis of β-catenin(GB15015).

Sample: Human esophageal carcinoma,4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

03 GB15015-catenin_ZCR17021_d1000_M stomach_40.0x_IHC.png

IHC analysis of β-catenin(GB15015).

Sample: Mouse stomach,4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

04 GB15015_-catenin_ZCR17021_d1000_R stomach_40.0x_IHC.png

IHC analysis of β-catenin(GB15015).

Sample: Mouse stomach,4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

Storage

Storage             Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB15015-50
: 10 Items
Hurry! only 10 items left in stock.

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