Anti-Vinculin Mouse mAb, Primary Antibody

Anti-Vinculin Mouse mAb, Primary Antibody

AO-03-GB121328-50

Anti-Vinculin Mouse mAb

Volume : 50 µL
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Anti-Vinculin Mouse mAb

Protein full name

Vinculin

Synonyms

Metavinculin, Vcl, CMD1W, CMH15, MVCL, MV, VINC

Immunogen

Recombinant protein corresponding to Human Vinculin

Uniprot ID

P18206

Isotype

IgG2a,k

Purity

Affinity purification

Predicted MW. / Observed MW.

117 kDa / 124 kDa

Applications

WB

Human, Mouse ,Rat

1: 500-1: 1000

lung , NIH/3T3 , uterine

Background

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion.

Images

01 GB121328_Vinculin_3E9H5_d1000_WB_WM.png

 WB analysis of Vinculin(GB121328).

Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB121328-50
: 10 Items
Hurry! only 10 items left in stock.

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