Recombinant Anti-CD34 antibody (Rabbit mAb), Primary Antibody

Recombinant Anti-CD34 antibody (Rabbit mAb), Primary Antibody

AO-03-GB15013-50

Recombinant Anti-CD34 antibody (Rabbit mAb), Primary Antibody

Volume : 50 µL
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€299.00
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Recombinant Anti-CD34 antibody (Rabbit mAb)

Protein full name

Hematopoietic progenitor cell antigen CD34

Synonyms

CD34, HPCA1, Mucosialin, Cluster designation 34, cluster of differentiation 34, CD34 antigen, CD34 molecule

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CD34

Uniprot ID

P28906, Q64314

Isotype

IgG

Purity

Affinity purification

Subcellular location

Membrane


Applications

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

kidney, liver cancer, liver


Background

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources.


Images

01 GB15013_CD34_M015_d100_H kidney_40.0x_IHC_WM.png

IHC analysis of CD34(GB15013).

Sample: Human kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB15013_CD34_M015_d100_liver cancer_20.0x_IHC_WM.png

IHC analysis of CD34(GB15013).

Sample: Human liver cancer, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03 GB15013_CD34_M015_d100_M liver_40.0x_IHC_WM.png

IHC analysis of CD34(GB15013).

Sample: Mouse liver, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04 GB15013_CD34_M015_d100_M lung_40.0x_IHC_WM.png

IHC analysis of CD34(GB15013).

Sample: Mouse lung, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05 GB15013_CD34_M015_d100_R liver_20.0x_IHC_WM.png

IHC analysis of CD34(GB15013).

Sample: Rat liver, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06 GB15013_CD34_M015_d100_R lung_40.0x_IHC_WM.png

IHC analysis of CD34(GB15013).

Sample: Rat lung, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.


Storage

Storage             Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

: AO-03-GB15013-50
: 10 Items
Hurry! only 10 items left in stock.

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