Anti-Aggrecan Rabbit pAb, Primary Antibody

Anti-Aggrecan Rabbit pAb, Primary Antibody

AO-03-GB11373-50

Anti-Aggrecan Rabbit pAb, Primary Antibody

Volume : 50 µL
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Anti-Aggrecan Rabbit pAb

Protein full name

Aggrecan core protein

Synonyms

ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, Aggrecan

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Aggrecan

Uniprot ID

Q61282

Isotype

IgG

Purity

Affinity purification

Subcellular location

Extracellular matrix

Applications

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

bone

Background

Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the lectican (chondroitin sulfate proteoglycan) family. This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

Images

01 GB11373_ Aggrecan_d500_H bone_40x_IHC.jpg

IHC analysis of Aggrecan(GB11373).

Sample: Human bone,4% PFA (G1101) 12-24h.

Antigen retrieval:citrate buffer (H 6.0) (G1201)，98℃,20 min.

Primary antibody:1: 500, 4℃ overnight.

Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

02 GB11373_ Aggrecan_d1000_H bone_40x_IHC.jpg

IHC analysis of Aggrecan(GB11373).

Sample: Human bone,4% PFA (G1101) 12-24h.

Antigen retrieval:citrate buffer (H 6.0) (G1201)，98℃,20 min.

Primary antibody:1: 1000, 4℃ overnight.

Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.
image.png

IHC analysis of Aggrecan (GB11373).

Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
image.png

IHC analysis of Aggrecan (GB11373).

Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
image.png

IHC analysis of Aggrecan (GB11373).

Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

01 GB11373_170901_Aggrecan_742_74217071902 M bone d500_40.0x_IHC.jpg

IHC analysis of Aggrecan (GB11373).

Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

02 GB11373_170901_Aggrecan_742_74217071902 R bone d500_40.0x_IHC.jpg

IHC analysis of Aggrecan (GB11373).

Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

01 GB11373_Aggrecan_20220516 M bone d500_30.0x_IF.jpg

IF analysis of Aggrecan (GB11373).

Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

02 GB11373_Aggrecan_20220516 R bone d500_30.0x_IF.jpg

IF analysis of Aggrecan (GB11373).

Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB11373-50
: 10 Items
Hurry! only 10 items left in stock.

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