Anti-IL-17 Rabbit pAb, Primary Antibody

Anti-IL-17 Rabbit pAb, Primary Antibody

AO-03-GB11110-1-50

Anti-IL-17 Rabbit pAb

Volume : 50 µL
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Anti-IL-17 Rabbit pAb

Protein full name

Interleukin-17A

Synonyms

IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse IL-17A

Uniprot ID

Q62386

Isotype

IgG

Purity

Affinity purification

Subcellular location

Secreted

Predicted MW. / Observed MW.

17 kDa / 17 kDa

Applications

WB

Mouse

1: 500-1: 1000

recombinant protein

IHC/IF

Human, Mouse, Rat

1: 500-1: 1400

hepatitis, liver

Background

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).

Images

01 GB11110-1_1107 IL17A_WB.jpg

WB analysis of IL17A (GB11110-1).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

02 GB11110-1_180201_IL-17A_1106_110617110802 M hepatitis d700_40.0x_IHC.jpg

IHC analysis of IL-17A (GB11110-1).

Sample: Mouse hepatitis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 700, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

03 GB11110-1_180201_IL-17A_1106_110617110802 M liver d700_40.0x_IHC.jpg

IHC analysis of IL-17A (GB11110-1).

Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 700, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB11110-1-50
: 10 Items
Hurry! only 10 items left in stock.

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