Recombinant Anti-VEGFA antibody (Rabbit mAb), Primary Antibody

Recombinant Anti-VEGFA antibody (Rabbit mAb), Primary Antibody

AO-03-GB15165-50

Recombinant Anti-VEGFA antibody (Rabbit mAb)

Volume : 50 µL
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Recombinant Anti-VEGFA antibody (Rabbit mAb)

Protein full name

Vascular endothelial growth factor A

Synonyms

VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGF, glioma-derived endothelial cell mitogen, MVCD1, VEGF165A

Immunogen

KLH conjugated Synthetic peptide corresponding to Human VEGFA

Uniprot ID

P15692

Isotype

IgG

Purity

Affinity purification

Subcellular location

Secreted

Applications

IHC/IF

Human, Mouse, Rat

1: 200-1: 400

kidney, stomach, liver cancer

Background

Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors.

Images

01 GB15165_20230517_VEGF_Recombinant_H kindey d250_20x_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Human kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

02 GB15165_20230517_VEGF_Recombinant_H leimyosarcoma d250_20x_IHC.jpg

IHC analysis of VEGFA (GB15165).

Sample: Human leiomyosarcoma (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 250, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

03 GB15165_20230517_VEGF_Recombinant_H liver cancer d250_20x_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Human liver cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

04 GB15165_20230517_VEGF_Recombinant_H stomach d250_20x_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Human stomach (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

05 GB15165_20230517_VEGF_Recombinant_M kindey d250_20x_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

06 GB15165_20230517_VEGF_Recombinant_M placenta d250_20x-2_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Mouse placenta (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

07 GB15165_20230517_VEGF_Recombinant_M subcutaneous nude-mouse model of 4T1 cell d250_20x_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Mouse subcutaneous nude-mouse model of 4T1 cell (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

08 GB15165_20230517_VEGF_Recombinant_M subcutaneous nude-mouse model of 4T1 cell d250_20x-2_IHC.jpg

 IHC analysis of VEGFA (GB15165).
Sample: Mouse subcutaneous nude-mouse model of 4T1 cell (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
09 GB15165_VEGFA_d400_R kidney_20.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.
10 GB15165_VEGFA_d400_R kidney_20.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.
11 GB15165_VEGFA_d400_R kidney_40.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.
12 GB15165_VEGFA_d400_R kidney_20.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.
13 GB15165_VEGFA_d400_R kidney_40.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.
14 GB15165_VEGFA_d400_R kidney_40.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.
15 GB15165_VEGFA_d400_R lung_40.0x_IHC_WM.png

IHC analysis of VEGFA(GB15165).

Sample: Rat lung, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 400, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB15165-50
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Hurry! only 10 items left in stock.

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