Anti-Caspase-3 Rabbit pAb, Primary Antibody, Primary Antibody

Anti-Caspase-3 Rabbit pAb, Primary Antibody, Primary Antibody

AO-03-GB11767C-50

Anti-Caspase-3 Rabbit pAb, Primary Antibody

Volume : 50 µL
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Anti-Caspase-3 Rabbit pAb

Protein full name

Caspase-3

Synonyms

Casp3, CASP3, CPP32, CPP32B, SCA-1, Caspase 3

Immunogen

Recombinant protein corresponding to Mouse Caspase-3

Uniprot ID

P70677

Isotype

IgG

Purity

Affinity purification

Predicted MW. / Observed MW.

31 kDa / 17/35 kDa

Applications

Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 500-1: 1000

liver, lung, brain

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

Images

01 GB11767C_2491 Caspase3_WB.png

WB analysis of Caspase-3(GB11767C).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02.png

WB analysis of Caspase-3(GB11767C).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

03 GB11767C_Caspase-3_d300_WB.png

WB analysis of Caspase-3(GB11767C).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB11767C-50
: 10 Items
Hurry! only 10 items left in stock.

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