Anti-NF-κB p65 Rabbit pAb
Product Information
|
Protein full name |
Transcription factor p65 |
|
Synonyms |
RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit |
|
Immunogen |
Recombinant protein corresponding to Mouse NF-κB p65 |
|
Uniprot ID |
Q04207 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Nucleus |
|
Predicted MW. / Observed MW. |
65 kDa / 70 kDa |
Applications
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lung, thymus |
|
ICC/IF |
Human, Mouse, Rat |
1: 1500-1: 3000 |
HeLa, NIH/3T3, PC-12 |
Background
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.
Images
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Western blot analysis of NF-kB p65 (GB11997) at dilution of 1: 500 |
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Western blot analysis of NF-kB p65 (GB11997) at dilution of 1: 500 |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using NF-κB p65 (GB11997) at dilution of 1: 3000 Left: HeLa cells untreated Right: HeLa cells were treated with 20ng/mL TNFα for 20min |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cells using NF-κB p65 (GB11997) at dilution of 1: 3000 Left: NIH/3T3 cells untreated Right: NIH/3T3 cells were treated with 20ng/mL TNFα for 20min |
 |
Immunocytochemistry analysis of 4% paraformaldehyde-fixed PC-12 cells using NF-κB p65 (GB11997) at dilution of 1: 3000 Left: PC-12 cells untreated Right: PC-12 cells were treated with 20ng/mL TNFα for 20min |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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