Anti-alpha smooth muscle Actin Rabbit pAb
Product Information
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Protein full name |
Actin, aortic smooth muscle |
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Synonyms |
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46, SMA, a-sma, sma |
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Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Sarcomeric Alpha Actinin |
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Uniprot ID |
P62737 |
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Isotype |
IgG |
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Purity |
Affinity purification |
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Subcellular location |
Cytoplasm |
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Predicted MW. / Observed MW. |
42 kDa / 42 kDa |
Applications
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WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
heart, liver |
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IHC/IF |
Human, Mouse, Rat |
1: 300-1: 1000 |
breast cancer, heart, liver |
|
IEM |
Mouse |
1: 50-1: 100 |
aorta |
Background
Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation.
Images
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Western blot analysis of alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded Mouse heart using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded Rat lung using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraformaldehyde-fixed Human breast cancer using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed Mouse lung using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed Rat heart using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed Rat liver using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed Human para-cancer lung using COLIII (GB11629) (green) + Collagen I (GB11022-3) (red) + alpha smooth muscle Actin (GB111364) (yellow) + CD31 (GB12064) (orange) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed Human lung cancer using alpha smooth muscle Actin(GB111364) (green) + CD31(GB12064) (spred) + CD3(GB11014) (pink) at dilution of 1: 300 |
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Immunoelectron microscopy analysis of LR white resin-embedded mouse aorta using alpha smooth muscle Actin (GB111364) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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