Recombinant Anti-beta Actin antibody (Mouse mAb)
Product Information
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Protein full name |
Actin, cytoplasmic 1 |
|
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
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Immunogen |
KLH conjugated synthetic peptidecorresponding to Mouse beta-Actin |
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Uniprot ID |
P60710 |
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Isotype |
IgG1 |
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Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
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Predicted MW. / Observed MW. |
42 kDa / 42 kDa |
Applications
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WB |
Human, Mouse, Rat |
1: 3000-1: 5000 |
lung, brain |
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 1000 |
NIH/3T3, rectum, liver, colon, stomach |
|
ICC/IF |
Human, Mouse, Rat |
1: 300-1: 1000 |
NIH/3T3, A549, HeLa. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.
Images
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WB analysis of beta Actin (GB15001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse rectum using β-Actin (GB15001) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using β-Actin (GB15001) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human colon using β-Actin (GB15001) at dilution of 1: 1000 |
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Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15001) at dilution of 1: 1000 |
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Immunocytochemistry analysis of 4% paraformaldehyde fixed A549 cells using β-Actin (GB15001) at dilution of 1: 1000 |
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ICC analysis of beta Actin(GB15001) red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody:Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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