Anti-ZO1 tight junction protein Mouse mAb, Primary Antibody

Anti-ZO1 tight junction protein Mouse mAb, Primary Antibody

AO-03-GB12195-50

Anti-ZO1 tight junction protein Mouse mAb

Volume : 50 µL
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€299.00
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Product Information

Protein full name

Tight junction protein ZO-1

Synonyms

TJP1, ZO-1, Tight junction protein 1

Immunogen

KLH conjugated Synthetic peptide corresponding to Human ZO-1

Uniprot ID

Q07157

Isotype

IgG2a,k

Purity

Affinity purification

Subcellular location

Cytoplasm, Cell membrane

Applications

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Mouse, Rat

1: 200-1: 800

colon, kidney

Background

Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton.The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation.Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells.Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling.With Tjp2 and TJjp3, participates in the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus.

Images

01 GB12195_ZO1 tight junction protein_7B1E23_7B1E23 20240807_d200_M small intestine_30.0x_IHC_WM.jpg

IHC analysis of ZO1 tight junction protein(GB12195).

Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

02 GB12195_ZO1 tight junction protein_7B1E23_7B1E23 20240807_d200_M kidney_40.0x_IHC_WM.jpg

IHC analysis of ZO1 tight junction protein(GB12195).

Sample: Mouse kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

03 GB12195_ZO1 tight junction protein_7B1E23_7B1E23 20240807_d200_M kidney_40.0x_IHC_WM.jpg

IHC analysis of ZO1 tight junction protein(GB12195).

Sample: Mouse kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

04 GB12195_ZO1 tight junction protein_7B1E23_7B1E23 20240807_d200_M small intestine_20.0x_IHC_WM.jpg

IHC analysis of ZO1 tight junction protein(GB12195).

Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

05 GB12195_ZO1 tight junction protein_7B1E23_7B1E23 20240807_d200_M small intestine_30.0x_IHC_WM.jpg

IHC analysis of ZO1 tight junction protein(GB12195).

Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

06 GB12195_ZO1 tight junction protein_7B1E23_7B1E23 20240807_d200_R colon_20.0x_IHC_WM.jpg

IHC analysis of ZO1 tight junction protein(GB12195).

Sample: Rat colon, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

07 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_H colon cancer_20.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Human colon cancer, 4% PFA (G1101) 12-24h.

Antigen retrieval:Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

08 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_H colon cancer_20.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Human colon cancer, 4% PFA (G1101) 12-24h.

Antigen retrieval:Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

09 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_H kidney_30.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Human kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

10 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_H kidney_40.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Human kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

11 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_M small intestine_40.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

12 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_R colon_30.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Rat colon, 4% PFA (G1101) 12-24h.

Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

13 GB12195_ZO1 tight junction protein_red_7B1E23_7B1E23 20240807_d200_R small intestine_20.0x_IF_WM.jpg

IF analysis of ZO1 tight junction protein(GB12195)red.

Sample: Rat small intestine, 4% PFA (G1101) 12-24h.

Antigen retrieval:Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

Storage

Storage             Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB12195-50
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Hurry! only 10 items left in stock.

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