Anti-Integrin beta 1/CD29 Rabbit pAb
Product Information
|
Protein full name |
Integrin beta-1 |
|
Synonyms |
CD29, FNRB, GPIIA, Integrin beta 1, ITB1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to mouse CD29 |
|
Uniprot ID |
P09055 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
|
Predicted MW. / Observed MW. |
88 kDa / 120-150 kDa |
Applications
|
WB |
Mouse, Rat |
1: 1000-1: 2000 |
kidney,liver,spleen,heart |
|
IHC/IF |
Mouse, Rat |
1: 500-1: 1000 |
colon, kidney |
Background
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen.Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen.Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin.Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin.Integrin alpha-5/beta-1 is a receptor for fibrinogen.Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin.Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion.
Images
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WB analysis of CD29(GB115629). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of CD29(GB115629). Sample:Mouse colon ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98℃ 20min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CD29(GB115629). Sample:Mouse kidney ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98℃ 20min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CD29(GB115629). Sample:Mouse liver ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98℃ 20min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CD29(GB115629). Sample:Rat colon ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98℃ 20min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
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|
IHC analysis of CD29(GB115629). Sample:Rat kidney ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98℃ 20min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of CD29(GB115629). Sample:Rat liver ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98℃ 20min.Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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