Anti-Phospho-H2A.X (S139) Rabbit pAb
Product Information
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Protein full name |
Histone H2AX |
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Synonyms |
H2a/x, Histone H2A.X, H2AFX, H2AX, H2A histone family member X, γ-H2AX |
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Immunogen |
KLH conjugated Synthetic phosphopeptide corresponding to Human gamma H2A.X (phospho S139) |
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Uniprot ID |
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Isotype |
IgG |
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Purity |
Affinity purification |
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Subcellular location |
Nucleus |
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Predicted MW. / Observed MW. |
15 kDa / 15 kDa |
Applications
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WB |
Human, Mouse, Rat |
1: 100-1: 500 |
A549, L929, PC12 |
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IHC/IF |
Human, Mouse, Rat |
1: 100-1: 500 |
spleen, testis |
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ICC/IF |
Human, Mouse, Rat |
1: 100-1: 500 |
A549, L929, PC12 |
Background
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.
Images
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Western blot analysis of γ-H2αX (GB111841) at dilution of 1: 100 |
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using H2AX (GB111841) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin-embedded rat spleen using H2AX (GB111841) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111841) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin-embedded rat testis using H2AX (GB111841) at dilution of 1: 200 |
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A |
B |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed A549 cell using H2AX (GB111841) at dilution of 1: 200 A: A549 cell-untreated. B: A549 cell-treated with UV irradiation for 30 min. |
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A |
B |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed L929 cell using H2AX (GB111841) at dilution of 1: 200 A: L929 cell-untreated. B: L929 cell-treated with UV irradiation for 30 min. |
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A |
B |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed PC-12 cell using H2AX (GB111841) at dilution of 1: 200 A: PC-12 cell-untreated. B: PC-12 cell-treated with UV irradiation for 30 min |
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Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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