Recombinant Anti-p21 antibody (Rabbit mAb)
   

Recombinant Anti-p21 antibody (Rabbit mAb)

Antibodies Oncology
AO-03-GB155313-100
CDKN1A,CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1,p21,cyclin-dependent kinase inhibitor 1A,cyclin dependent kinase inhibitor 1A,Cyclin-dependent kinase inhibitor 1
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 1

Synonyms

CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, p21, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase inhibitor 1A

Uniprot ID

P39689

Immunogen

Recombinant protein corresponding to Mouse p21

Isotype

IgG, κ

Clone Number

SB249

Predicted MW./Observed MW.

19 kDa / 20 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Mouse 1: 500-1: 1000 NIH/3T3, UV-irradiated mouse skin
IHC/IF Mouse 1: 1000-1: 3000 skin, Ultraviolet irradiation of the skin for 16 hours, testis



Background

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.



Images

01p7mOo8eHUCkin.jpg

 WB analysis of p21(GB155313).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02JRlYUGPfmzqEk.jpg

 IHC analysis of p21(GB155313).
Sample: Mouse skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

03ZTiocM5uRz9Hi.jpg

 IHC analysis of p21(GB155313).
Sample: Mouse subcutaneous nude-mouse model of 4T1 cell, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04uazXWsmkYtjgx.jpg

 IHC analysis of p21(GB155313).
Sample: Mouse subcutaneous nude-mouse model of B16 cell, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05RwloxAKZ5JVog.jpg

 IHC analysis of p21(GB155313).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06zOW58KByIt5b6.jpg

 IHC analysis of p21(GB155313).
Sample: Mouse Ultraviolet irradiation of the skin for 16 hours, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

07z9LGA3QLSVX6x.jpg

 IF analysis of p21(GB155313)red.
Sample: Mouse skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08eHsqgkiS8Bp29.jpg

 IF analysis of p21(GB155313)red.
Sample: Mouse subcutaneous nude-mouse model of 4T1 cell, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

093PfPyIL1tALES.jpg

 IF analysis of p21(GB155313)red.
Sample: Mouse subcutaneous nude-mouse model of B16 cell, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

10pBULvu6JqeHc2.jpg

 IF analysis of p21(GB155313)red.
Sample: Mouse Ultraviolet irradiation of the skin for 16 hours, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11FWrSTjWmmdRB9.jpg

 IF analysis of p21(GB155313)red.
Sample: Mouse Ultraviolet irradiation of the skin for 16 hours, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB155313-100
: 10 Items
Hurry! only 10 items left in stock.

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