Recombinant Anti-p21 antibody (Mouse mAb)

Recombinant Anti-p21 antibody (Mouse mAb)

Antibodies Oncology
AO-03-GB15153-50
CIP1,p21CIP1,MDA-6,15153,Rpa(,p-21,CDKN1,SDI1,GB15153,CDKN1A,GB15153100,CAP20,WAF1,p 21,P21,p21,cyclin-dependent kinase inhibitor 1A,cyclin dependent kinase inhibitor 1A,Cyclin-dependent kinase inhibitor 1,P21抗体
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 1

Synonyms

CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, p21, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase inhibitor 1A

Uniprot ID

P38936

Immunogen

Recombinant protein corresponding to Human p21

Isotype

IgG1, κ

Clone Number

SB51

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 cervical carcinoma, testis



Background

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.



Images

013VShCnOgGLiGJ.jpg

 IHC analysis of p21(GB15153).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

02Qw5AZIHBZ1Zxz.jpg

 IHC analysis of p21(GB15153).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB15153-50
: 10 Items
Hurry! only 10 items left in stock.

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