Anti-CDKN2A/p14ARF Rabbit pAb
   

Anti-CDKN2A/p14ARF Rabbit pAb

Antibodies Oncology
AO-03-GB115589-50
CDKN2,115589,CDKN-2,p 14,GB115589100,CDK4 inhibitor p16 INK4,CDKN 2,p 14ARF,CDK4 inhibitor p16INK4,CDK4I,ARF,p-14ARF,p-14,CDKN2A,CDKN 2A,CDKN-2A,GB115589,Tumor suppressor ARF
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Product Information


Protein full name

Tumor suppressor ARF

Synonyms

ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2

Uniprot ID

Q64364

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse p14ARF

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Mitochondrion



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human 1: 150-1: 600 lung, liver, tonsil



Background

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity.



Images

01lKQBxati7HA6e.jpg

 IHC analysis of CDKN2A/p14ARF(GB115589).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

027S9SOzJSJJLss.jpg

 IHC analysis of CDKN2A/p14ARF(GB115589).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03cM2dYgboSuVLd.jpg

 IHC analysis of CDKN2A/p14ARF(GB115589).
Sample: Human esophageal cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04WN5z4tmic2Ixv.jpg

 IHC analysis of CDKN2A/p14ARF(GB115589).
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05tqcbj3DrfB5Ja.jpg

 IHC analysis of CDKN2A/p14ARF(GB115589).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

063p8dcuhdO3C7D.jpg

 IHC analysis of CDKN2A/p14ARF(GB115589).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115589-50
: 10 Items
Hurry! only 10 items left in stock.

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