Recombinant Anti-BrdU antibody (Mouse mAb)

Recombinant Anti-BrdU antibody (Mouse mAb)

Antibodies Oncology
AO-03-GB15051-100
5-Bromo-2′-deoxyuridine; 5-BrdU,GB15051,Proliferation Marker; 5-Bromo-2-deoxyUridine,Bromodeoxyuridine,15051,Bromodeoxyuridine
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Product Information


Protein full name

Bromodeoxyuridine

Synonyms

Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse BrdU

Isotype

IgG1, κ

Clone Number

SB153

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Species independent 1: 500-1: 1000 brain,spleen



Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.



Images

01jQwQAE7nWO9Ke.jpg

 IHC analysis of BrdU(GB15051).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

02D8BJcC0oRwgYI.jpg

 IHC analysis of BrdU(GB15051).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB15051-100
: 10 Items
Hurry! only 10 items left in stock.

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