Recombinant Anti-Ki67 antibody (Mouse mAb)

Recombinant Anti-Ki67 antibody (Mouse mAb)

Antibodies Oncology
AO-03-GB151142-100
KIA,151142,MKI67,KI 67,RKa(,PPP1R105,GB151142100,marker of proliferation Ki-67,MIB-1,KI-67,GB151142,Proliferation marker protein Ki-67,GB13030-2,Ki67染色
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Product Information


Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA,MIB-1, PPP1R105

Uniprot ID

P46013

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Ki67

Isotype

IgG1, κ

Clone Number

SB43

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 colon cancer, colon



Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .



Images

01uXtmTh1Tq8Yko.jpg

 IHC analysis of Ki67(GB151142).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

02a7EMuJzLIaFrR.jpg

 IHC analysis of Ki67(GB151142).
Sample: Mouse colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

037NV0oqUe0ZiOW.jpg

 IHC analysis of Ki67(GB151142).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB151142-100
: 10 Items
Hurry! only 10 items left in stock.

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