Product Information
|
Protein full name |
Protein BTG4 |
|
Synonyms |
B cell translocation gene 4, BTG family member 4, PC3B, Protein BTG4, Protein PC3b |
|
Uniprot ID |
O70552 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse BTG4 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus, Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Rat | 1: 800-1: 2400 | colon |
| ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
Adapter protein that bridges CNOT7, a catalytic subunit of the CCR4-NOT complex, to EIF4E, and facilitates maternal mRNAs decay during the maturation of oocytes and in the fertilized egg. It is therefore required for the maternal-zygotic transition (MZT), zygotic cleavage and initiation of embryonic development.
Images
|
|
IHC analysis of BTG4(GB114989). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
ICC analysis of BTG4(GB114989)red. Sample: Hela, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
Français
English
