Anti-p21 Rabbit pAb

Anti-p21 Rabbit pAb

Antibodies Oncology
AO-03-GB115313-50
GB115313,CIP1,p21CIP1,MDA-6,p-21,CDKN1,SDI1,115313,CDKN1A,p21,CAP20,GB115313100,WAF1,p 21,P21,cyclin-dependent kinase inhibitor 1A,cyclin dependent kinase inhibitor 1A,Cyclin-dependent kinase inhibitor 1,P21抗体
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 1

Synonyms

CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, p21, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase inhibitor 1A

Uniprot ID

P39689

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse p21

Isotype

IgG

Predicted MW./Observed MW.

19 kDa / 20 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse 1: 300-1: 600 293T, MCF7, T47D, NIH3T3



Background

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.



Images

01zM266STLloLxn.jpg

 WB analysis of p21(GB115313).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02HulVIX8cGAT27.jpg

 WB analysis of p21(GB115313).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115313-50
: 10 Items
Hurry! only 10 items left in stock.

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