Anti-CCNI2 Rabbit pAb
   

Anti-CCNI2 Rabbit pAb

Antibodies Oncology
AO-03-GB115090-50
CCNI-2,GB115090,CCNI2,115090,CCNI 2,Cyclin I2,member 2,cyclin I family,Cyclin-I2
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Product Information


Protein full name

Cyclin-I2

Synonyms

CCNI2, cyclin I family, member 2, Cyclin I2

Uniprot ID

Q6ZMN8

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CCNI2

Isotype

IgG

Predicted MW./Observed MW.

41 kDa / 40 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 800-1: 1500 Hep G2, 293T, MCF7
IHC/IF Human, Mouse, Rat 1: 500-1: 2000 liver, kidney, brain, spleen
ICC/IF Mouse 1: 100-1: 600 NIH-3T3



Background

Orphan receptor.



Images

01NC6qV4tbJBTHS.jpg

 WB analysis of CCNI2(GB115090).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02D2mggQxhkKe8i.jpg

 IHC analysis of CCNI2(GB115090).
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03ksrrcDQYFcJdl.jpg

 IHC analysis of CCNI2(GB115090).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04yPm7ywyj0w0oc.jpg

 IHC analysis of CCNI2(GB115090).
Sample: Rat Brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05V5aJK4eYJb0VB.jpg

 IHC analysis of CCNI2(GB115090).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06pUpXQmVZczIo6.jpg

 IHC analysis of CCNI2(GB115090).
Sample: Mouse liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07AfX6ltEqjZMcF.jpg

 ICC analysis of CCNI2(GB115090)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115090-50
: 10 Items
Hurry! only 10 items left in stock.

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