Anti-KLHL13 Rabbit pAb

Anti-KLHL13 Rabbit pAb

Antibodies Oncology
AO-03-GB114919-50
KLHL-13,KLHL 13,GB114919,kelch like 13 (Drosophila),114919,KIAA1309,Kelch like protein 13,KLHL13,BKLHD2,GB114919100,Kelch-like protein 13
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Product Information


Protein full name

Kelch-like protein 13

Synonyms

BKLHD2, kelch like 13 (Drosophila), Kelch like protein 13, KIAA1309

Uniprot ID

Q80TF4

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse KLHL13

Isotype

IgG

Predicted MW./Observed MW.

74 kDa / 71 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 800-1: 1200 placenta,testis
ICC/IF Mouse 1: 100-1: 300 NIH-3T3



Background

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.



Images

013VUF4OxwRkbLI.jpg

 WB analysis of KLHL13(GB114919).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02vRFXNPqKQMa78.jpg

 WB analysis of KLHL13(GB114919).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03xvQcR1ojUJhQm.jpg

 ICC analysis of KLHL13(GB114919)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114919-50
: 10 Items
Hurry! only 10 items left in stock.

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