Anti-MTBP Rabbit pAb

Anti-MTBP Rabbit pAb

Antibodies Oncology
AO-03-GB114421-50
MDM2BP,114421,Mdm2 binding protein,GB114421,hMTBP,MTBP,Mdm2-binding protein
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Product Information


Protein full name

Mdm2-binding protein

Synonyms

hMTBP, Mdm2 binding protein, MDM2BP, MTBP

Uniprot ID

Q8BJS8

Immunogen

Recombinant protein corresponding to Mouse MTBP

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human 1: 1000-1: 2000 kidney, lung cancer, colon cancer



Background

May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells.



Images

01GjOcaaV0f1xaB.jpg

 IHC analysis of MTBP(GB114421).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02jDZYlMgr4vly2.jpg

 IHC analysis of MTBP(GB114421).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03SpVLNPajwMVpP.jpg

 IF analysis of MTBP(GB114421)red.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114421-50
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