Anti-SMC3 Rabbit pAb

Anti-SMC3 Rabbit pAb

Antibodies Oncology
AO-03-GB114015-50
114015,Bamacan,SMC-3,CDLS3,BAM,CSPG6,GB114015,HCAP,BMH,SMC 3,SMC3L1,SMC3,SMC protein 3,Structural maintenance of chromosomes protein 3
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Product Information


Protein full name

Structural maintenance of chromosomes protein 3

Synonyms

BAM, Bamacan, BMH, CDLS3, CSPG6, HCAP, SMC 3, SMC protein 3, SMC3, SMC3L1

Uniprot ID

Q9CW03

Immunogen

Recombinant protein corresponding to Mouse SMC3

Isotype

IgG

Predicted MW./Observed MW.

142 kDa / 142 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 brain, kidney



Background

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.



Images

019u73IDbkiYOS7.jpg

 WB analysis of SMC3(GB114015).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02lnij4UVd5CqH7.jpg

 WB analysis of SMC3(GB114015).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

033fTwlpCsLT3Gv.jpg

 WB analysis of SMC3(GB114015).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114015-50
: 10 Items
Hurry! only 10 items left in stock.

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