Anti-Rad9 Rabbit pAb

Anti-Rad9 Rabbit pAb

Antibodies Oncology
AO-03-GB113416-50
Rad-9,Rad9-like protein,RAD9 homolog A (S. pombe),DNA repair exonuclease rad9 homolog A,Rad9,RAD9A,GB113416,RAD9,113416,hRAD9,Rad 9,Cell cycle checkpoint control protein RAD9A
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Product Information


Protein full name

Cell cycle checkpoint control protein RAD9A

Synonyms

hRAD9, RAD9, RAD9 homolog A (S. pombe), RAD9A, DNA repair exonuclease rad9 homolog A, Rad9-like protein

Uniprot ID

Q9Z0F6

Immunogen

Recombinant protein corresponding to Mouse Rad9

Isotype

IgG

Predicted MW./Observed MW.

42 kDa / 55 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 800-1: 1500 lung, testis



Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.



Images

01BCLDjkJUlefZf.jpg

 WB analysis of Rad9(GB113416).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02RFgzWXVez7ynN.jpg

 WB analysis of Rad9(GB113416).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03dIM4Gz0H9Vn4A.jpg

 WB analysis of Rad9(GB113416).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113416-50
: 10 Items
Hurry! only 10 items left in stock.

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