Product Information
|
Protein full name |
Septin-7 |
|
Synonyms |
CDC10, CDC10 protein homolog, CDC3, Nbla02942, SEPT7, SEPT7A, septin 7 |
|
Uniprot ID |
O55131 |
|
Immunogen |
Recombinant protein corresponding to Mouse SEPT7 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
51 kDa / 51 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 500-1: 800 | brain |
| ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.
Images
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WB analysis of SEPT7(GB113385). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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ICC analysis of SEPT7(GB113385)red. Sample: Hela, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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