Anti-14-3-3 sigma/SFN Rabbit pAb

Anti-14-3-3 sigma/SFN Rabbit pAb

Antibodies Oncology
AO-03-GB113320-50
Epithelial cell marker protein 1,14-3-3 sigma/SFN,YWHAS,HME1,1433sigma,GB113320,113320,Mkrn3,SFN,1433 sigma,stratifin,Er,Mme1,14 3 3,1433,14-3-3 protein sigma,免疫球蛋白
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Product Information


Protein full name

14-3-3 protein sigma

Synonyms

HME1, SFN, stratifin, YWHAS, Epithelial cell marker protein 1, Er, HME1, Mkrn3, Mme1

Uniprot ID

O70456

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse 14-3-3 sigma/SFN

Isotype

IgG

Predicted MW./Observed MW.

28 kDa / 28 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 300-1: 600 Skin, brain, lung



Background

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.



Images

01Rh1xg21jkYAiz.jpg

 WB analysis of 14-3-3 sigma/SFN(GB113320).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02qPWmMJg8ZkWzF.jpg

 WB analysis of 14-3-3 sigma/SFN(GB113320).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03uo7IFsnup1dph.jpg

 WB analysis of 14-3-3 sigma/SFN(GB113320).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113320-50
: 10 Items
Hurry! only 10 items left in stock.

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